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Cloning and Expression
Cell Culture / Tissue Culture
Cell-Based Assays
Cell Staining Dyes
Phalloidin Stains and Reagents
Acti-stain™ 488 phalloidin
Acti-stain™ 488 phalloidin from Cytoskeleton, Inc.
Be the first to write a review!
Citations:
(31)
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Cytoskeleton, Inc. for
Acti-stain™ 488 phalloidin
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Citations
Reviews
Product Specs
Item
Acti-stain™ 488 phalloidin
Company
Cytoskeleton, Inc.
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
PHDG1-A
Quantity
1 x 500 µl (300 slides)
Brand/Trademark
Cytoskeleton, Inc
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Cytoskeleton, Inc.
1830 S. Acoma St.
Denver
,
Colorado
80223
United States
Phone
: 303 322-2254
303 322-2257
Company Profile
Website:
http://cytoskeleton.com/
Citations (31)
(1)
N-WASP-dependent branched actin polymerization attenuates B-cell receptor signaling by increasing the molecular density of receptor clusters
Elife
December 12, 2023
Anshuman Bhanja, Margaret K. Seeley-Fallen, Michelle Lazzaro, Arpita Upadhyaya, Wenxia Song
polyclonal),
Thermo Fisher Scientific
,
A-11035
,IF (
1:200
) Other,
Acti-stain-488 Phalloidin
,
Cytoskeleton
,
PHDG1-A
,IF (200 nM) Other,
Acti-stain-555 Phalloidin
,
Cytoskeleton
,
PHDH1-A
,IF (200 nM) Antibody,Rabbit IgG anti-
mouse
(2)
The human acetylcholinesterase C-terminal T30 peptide activates neuronal growth through alpha 7 nicotinic acetylcholine receptors and the mTOR pathway
Scientific Reports
July 15, 2023
Alexandru Graur, Patricia Sinclair, Amanda K. Schneeweis, Daniel T. Pak, Nadine Kabbani
structural change (i.e.,
neurite
shape and growth) was performed using rhodamine phalloidin (
Cytoskeleton
PHDG1-A
). All morphometric
measures
were conducted and quantified using ImageJ (NIH, Bethesda, MD, USA) as described8.
(3)
ClC-7 drives intraphagosomal chloride accumulation to support hydrolase activity and phagosome resolution
Journal of Cell Biology
June 5, 2023
Jing Ze Wu, Mariia Zeziulia, Whijin Kwon, Thomas J. Jentsch, Sergio Grinstein, Spencer A. Freeman
Sigma-Aldrich (cat#: F4274). Other reagents include fluorescently labeled phalloidin (Acti-Stain; cat#:
PHDG1-A
;
Cytoskeleton Inc
.),
concanamycin A
(cat#:
C9705
;
Sigma-Aldrich
), nigericin (cat#:
ab120494
;
Abcam
),
(4)
Compound 13 Promotes Epidermal Healing in Mouse Fetuses via Activation of AMPK
Biomedicines
March 27, 2023
Kento Takaya, Keisuke Okabe, Shigeki Sakai, Noriko Aramaki-Hattori, Toru Asou, Kazuo Kishi
temperature (20–25 °C). The cells were then incubated overnight at 4 °C with
Acti-stain 488 phalloidin
(
PHDG1-A
;
Cytoskeleton, Inc
., Denver, CO, USA;
1:140
) for actin
staining
; after washing three times with PBST,
(5)
Functionalized TiCu/TiCuN coating promotes osteoporotic fracture healing by upregulating the Wnt/β-catenin pathway
Regenerative Biomaterials
January 17, 2023
Jia Tan, Ling Ren, Kai Xie, Lei Wang, Wenbo Jiang, Yu Guo, Yongqiang Hao
paraformaldehyde for 40 min and washed with PBS again. The discs were then stained with
Acti-stain 555 phalloidin
(
PHDG1-A
;
Cytoskeleton
, Shanghai, China) solution for 40 min, washed with PBS three times and finally stained
(6)
Actin cable formation and epidermis-dermis positional relationship during complete skin regeneration
Scientific reports
September 23, 2022
Takaya K, Okabe K, Ishigami A, Imbe Y, Kanazawa H, Sakai S, Aramaki-Hattori N, Kishi K.
h at room temperature. For actin
staining
, the cells were incubated with
Acti-stain 488 phalloidin
(
PHDG1-A
;
Cytoskeleton, Inc
., Denver, CO, USA;
1:140
) overnight at 4 °C. After washing three times with PBST,
(7)
On‐Demand Chemomagnetic Modulation of Striatal Neurons Facilitated by Hybrid Magnetic Nanoparticles
Advanced Functional Materials
August 25, 2022
Rohini Thevi Guntnur, Nicolas Muzzio, Amanda Gomez, Sean Macias, Arturo Galindo, Arturo Ponce, Gabriela Romero
temperature. After washing 3 times,
actin cytoskeleton
fibers were stained with actin-stain 488 phalloidin (
PHDG1-a
,
Cytoskeleton, Inc
.). Followinig washing,
cell nucleus
counterstaining was performed by incubating with
(8)
DAPLE orchestrates apical actomyosin assembly from junctional polarity complexes
Journal of Cell Biology
May 2, 2022
Arthur Marivin, Rachel Xi-Yeen Ho, Mikel Garcia-Marcos
Fluor 647 (
1:400
; A21247). For experiments
staining
for F-actin, Alexa Fluor 488–conjugated phalloidin (
PHDG1-A
;
Cytoskeleton
) was included along secondary antibodies at 70 nM after drying of methanol stocks and
(9)
Wireless Force-Inducing Neuronal Stimulation Mediated by High Magnetic Moment Microdiscs
Advanced healthcare materials
March 1, 2022
Collier C, Muzzio N, Thevi Guntnur R, Gomez A, Redondo C, Zurbano R, Schuller IK, Monton C, Morales R, Romero G.
Antibody (
clone
TU-20, Alexa Fluor555 Conjugate,
CBL412A5
from
Sigma Aldrich
), actin-stain 488 phalloidin (
PHDG1-a
,
Cytoskeleton, Inc
.) and
Mouse Monoclonal Synaptophysin Antibody
(Alexa Fluor647 Conjugate,
NBP147483AF647
(10)
Protein Aggregation of NPAS3, Implicated in Mental Illness, Is Not Limited to the V304I Mutation
Journal of Personalized Medicine
October 23, 2021
Bobana Samardžija, Aristea Pavešić Radonja, Beti Zaharija, Mihaela Bergman, Éva Renner, Miklós Palkovits, Gordana Rubeša, Nicholas J. Bradshaw
secondary antibody (
A11037
,
Thermo Fisher Scientific
, Waltham, MA, USA), fluorescently labelled phalloidin (
PHDG1-A
,
Cytoskeleton
, Denver, CO, USA) and DAPI (Merck) for 1 h, before 4 final washes (as above). Slides were
(11)
Two Novel Pathogenic Variants of TJP2 Gene and the Underlying Molecular Mechanisms in Progressive Familial Intrahepatic Cholestasis Type 4 Patients
Frontiers in Cell and Developmental Biology
August 24, 2021
Jia Tang et al.
LO2 cells were visualized by
confocal microscopy
using anti-F-actin (
1:500
;
Cytoskeleton, Inc
. Cat. #
PHDG1-A
) and anti-β-tubulin (
1:300
;
Abcam
, Cat. #
ab195883
). Detailed information regarding experimental operations
(12)
Microtissue Geometry and Cell‐Generated Forces Drive Patterning of Liver Progenitor Cell Differentiation in 3D
Advanced Healthcare Materials
April 23, 2021
Ian C. Berg, Erfan Mohagheghian, Krista Habing, Ning Wang, Gregory H. Underhill
anti-Ecadherin (1/50 from stock,
R&D Systems
AF748
), Actin-stain 488 phalloidin (7/1000 from stock,
Cytoskeleton
PHDG1-A
),
goat
anti-
ALB (1
/100 from stock,
Bethyl
A90-134A
),
goat
anti-CK19 (1/200 from stock,
Abcam
ab52625
).
(13)
Epithelial to Mesenchymal Transition Regulates Surface PD-L1 via CMTM6 and CMTM7 Induction in Breast Cancer
Cancers (Basel)
March 9, 2021
Malina Xiao et al.
CMTM6
(Sigma-Aldrich,
HPA026980
),
Acti-Stain 488 Phalloidin
(
Cytoskeleton, Inc
., Denver, CO, USA, #
PHDG1-A
) and Goat anti-Rabbit IgG Secondary Antibody, Alexa Fluor Plus 488 (
Thermofischer
Scientific, #
A32731
). 2.7.
(14)
Complement Factor H-Related 3 Enhanced Inflammation and Complement Activation in Human RPE Cells
Frontiers in immunology
January 1, 2021
Schäfer N, Rasras A, Ormenisan DM, Amslinger S, Enzmann V, Jägle H, Pauly D.
used to stain DNA and
acti-stain™ 488 phalloidin
( Figures 5D, E ,
Cytoskeleton
, Denver, CO, USA, #
PHDG1-A
) detected specifically
actin filaments
in ARPE-19 cells. Cells were covered with
fluorescence
mounting
(15)
NAIP-NLRC4-deficient mice are susceptible to shigellosis
eLife
October 19, 2020
Mitchell PS, Roncaioli JL, Turcotte EA, Goers L, Chavez RA, Lee AY, Lesser CF, Rauch I, Vance RE.
performed using Imaris. Reagents for
immunofluorescence
:
anti-Shigella
(
Abcam
,
ab65282
), 488 phalloidin (
PHDG1-A
,
Cytoskeleton Inc
), Alexfluor conjugated secondary antibodies (Invitrogen). To determine bacterial replication
(16)
Golgi Acidification by NHE7 Regulates Cytosolic pH Homeostasis in Pancreatic Cancer Cells
Cancer discovery
June 1, 2020
Galenkamp KMO, Sosicka P, Jung M, Recouvreux MV, Zhang Y, Moldenhauer MR, Brandi G, Freeze HH, Commisso C.
were incubated for 4 hours at room temperature with primary antibodies or
Acti-stain 488 phalloidin
(#
PHDG1-A
,
Cytoskeleton
) diluted in blocking buffer. Next, cells were washed three times in PBS and incubated
(17)
Some Ototoxic Drugs Destroy Cochlear Support Cells Before Damaging Sensory Hair Cells
Neurotoxicity Research
March 1, 2020
Dalian Ding, Jianhui Zhang, Haiyan Jiang, Weijun Xuan, Weidong Qi, Richard Salvi
stereocilia
of hair cells, some samples were labeled with
Acti-Stain 488 Phalloidin
(
1:100
, Cat. #
PHDG1-A
,
Cytoskeleton, Inc
.) or Acti-Stain Phalloidin 555 (Cat. #
PHDH1A
,
Cytoskeleton
). Nuclei were stained
(18)
Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent
Marine Drugs
February 8, 2020
Dhanak Gupta, Melissa Silva, Karolina Radziun, Diana C. Martinez, Christopher J. Hill, Julie Marshall, Vanessa Hearnden, Miguel A. Puertas-Mejia, Gwendolen C. Reilly
again washed twice in DPBS for 5 min and counter stained with 100 nM of
Acti-stain 488 phalloidin
(
PHDG1-A
,
Cytoskeleton, Inc
., Denver, CO, USA) (if needed) and 10 µg/mL DAPI in 1% BSA/DPBS for 15 min at RT.
(19)
Chronic Kidney Disease Increases Cerebral Microbleeds in Mouse and Man
Translational Stroke Research
February 1, 2020
Wei Ling Lau et al.
immunofluorescence
staining
using acti-stain 488 fluorescent phalloidin with DAPI nuclear stain (catalog#
PHDG1-A
,
Cytoskeleton Inc
., Denver, CO). Triplicate slides were done per group, and three frames were imaged
(20)
CaSR Induces Osteoclast Differentiation and Promotes Bone Metastasis in Lung Adenocarcinoma
Frontiers in oncology
January 1, 2020
Liu L, Fan Y, Chen Z, Zhang Y, Yu J.
X-100 for 5 min. The cells were then stained using the
Acti-stain™ 488 phalloidin
(
Cytoskeleton Inc
.
PHDG1-A
) at room temperature in the dark for 30 min. The formation of the actin ring was captured using the
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Acti-stain™ 488 phalloidin from Cytoskeleton, Inc.
Supplier Page
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Pricing Info
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Company Info
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